"The female was lost at a critical juncture in the wolves history. The shortage of females means the wolves, which have been on the island since the 1950s, may well go extinct. There is one other known female on the island who is believed to have mated, but the scientists won’t know that until get a glimpse of pups later this summe"
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Isle Royale wolf mystery solved, sadly
(Source: startribune.com)